مطالعه تأثیر یک دوره مصرف نانوکپسول سیاه دانه بر ساختار هیستوپاتولوژیک و سطوح فاکتور رشد اندوتلیال عروق بافت ریه موش‌های صحرایی در معرض نیتروز آمین کتون مشتق از تنباکو

Background and Objectives: Cigarette smoke contains carcinogenic compounds, including nicotine-derived nitrosamine ketone (NNK), which can activate vascular endothelial growth factor (VEGF) and increase the inflammation of the lung. Since the Nigella sativa plays an important role in treating cancer through the modification of cellular signaling pathways, the purpose of this study was determining the effect of a period of Nigella sativa nanocapsules consumption on the histopathological structure and VEGF levels of the lung tissue of the rats exposed to tobacco-derived nitrosamines ketone. Materials and Methods: In this experimental study, 45 Wistar male and female rats with the average weight and standard deviation of 103.83±23.48 grams were randomly divided into five groups of control, saline, NNK, supplement, and supplement+NNK. The NNK-induced groups received NNK subcutaneously once per week at a rate of 12.5 mg/kg/bw for 12 weeks. The supplement groups also received Nigella sativa nanocapsules for 12 weeks. Levels of VEGF in the homogenized lung tissue were measured by enzyme-linked immunosorbent assay (ELISA). One-way ANOVA and Tukey’s multiple comparisons test were used to analyze the data Results: Exposure to NNK significantly increased VEGF levels compared to the saline group (p=0.001). Meanwhile, a period of Nigella sativa  nanocapsules consumption significantly reduced the levels of VEGF in the supplement+NNK group compared to the NNK group (p=0.001). Conclusion: Considering the findings, it seems that Nigella sativa nanocapsules consumption can be helpful in cigarette smoke-induced inflammation by reducing VEGF levels . Key words: Nigella sativa nanocapsules, Vascular endothelial growth factor, Nitrosamine ketone, Tobacco, Histological structure, Rat.   Funding: This study was funded by University of Mazandaran. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Babol University of Medical Sciences approved the study (MUBABOL.HRI.REC.1395.109).   How to cite this article: Barzegari A, Mirdar Sh. Investigating the Effect of One Period of Nigella Sativa Nanocapsules Consumption on the Histopathological Structure and Vascular Endothelial Growth Factor Levels of the Lung Tissue of the Rats Exposed to Nicotine-Derived Nitrosamine Ketone. J Rafsanjan Univ Med Sci 2018; 17 (5): 435-46. [Farsi]